| Teams 1965 100 Mile Qualifying Race #1 |
|---|
| No. | Team | Driver | Mfg |
|---|---|---|---|
| 3 | Fox Racing | LeeRoy Yarbrough | Dodge |
| 4 | H.B. Bailey | H.B. Bailey | Pontiac |
| 7 | Holman-Moody Racing | Bobby Johns | Ford |
| 11 | Bondy Long | Ned Jarrett | Ford |
| 12 | Bobby Allison Motorsports | Bobby Allison | Ford |
| 14 | Sam Fletcher | Johnny Allen | Plymouth |
| 16 | Bud Moore Engineering | Darel Dieringer | Mercury |
| 19 | Herman Beam | J.T. Putney | Chevrolet |
| 29 | Holman-Moody Racing | Dick Hutcherson | Ford |
| 37 | Bub Strickler | Bub Strickler | Ford |
| 44 | Larry Hess | Larry Hess | Ford |
| 49 | GC Spencer Racing | G.C. Spencer | Ford |
| 55 | Lyle Stelter | Tiny Lund | Mercury |
| 60 | Bob Cooper | Doug Cooper | Ford |
| 61 | Bob Cooper | Barry Brooks | Pontiac |
| 64 | Langley Racing | Elmo Langley | Ford |
| 72 | Friedkin Enterprises | Jerry Grant | Ford |
| 76 | Larry Frank | Larry Frank | Ford |
| 79 | Harold Rhodes | Joe Penland | Chevrolet |
| 81 | Joe Keistler | Don Tilley | Dodge |
| 82 | Casper Hensley | Bunkie Blackburn | Pontiac |