| Teams for 2000 Daytona 250 |
|---|
| Car | Team | Driver | Mfg |
|---|---|---|---|
| 00 | Raynard McGlynn | Ryan McGlynn |  |
| 1 | Bob Keselowski | Dennis Setzer |  |
| 2 | Ultra Motorsports | Mike Wallace |  |
| 3 | Team Menard | Bryan Reffner | |
| 4 | Bobby Hamilton | Bobby Hamilton | |
| 6 | Richard Woodland | Rich Woodland, Jr. | |
| 7 | PF2 Motorsports | Morgan Shepherd | |
| 12 | Impact Motorsports | Carlos Contreras | |
| 14 | Tom Mitchell | Rick Crawford | |
| 15 | Billy Ballew | Geoffrey Bodine | |
| 16 | Ed Rensi | Jimmy Hensley | |
| 18 | Bobby Hamilton | Joe Ruttman | |
| 24 | Rick Hendrick | Jack Sprague | |
| 25 | Impact Motorsports | Randy Tolsma | |
| 26 | MB Motorsports | Jamie McMurray | |
| 27 | Mike Albernaz | Lonnie Rush, Jr. | |
| 31 | Brevak Racing | John Young | |
| 35 | George Starr | David Starr | |
| 41 | TKO Motorsports | Randy Renfrow | |
| 42 | McCray Racing | Jimmy Kitchens | |
| 43 | Richard Petty | Steve Grissom | |
| 46 | Dollar Racing | Rob Morgan | |
| 50 | Jack Roush | Greg Biffle | |
| 52 | Ken Schrader | Lyndon Amick | |
| 60 | Mike Addington | Andy Houston | |
| 66 | Phelon Motorsports | Rick Carelli | |
| 72 | MacDonald Motorsports | Randy MacDonald | |
| 73 | Sonntag Racing | B.A. Wilson | |
| 75 | Wayne Spears | Marty Houston | |
| 84 | Long Brothers Racing | Donnie Neuenberger | |
| 86 | Team 34 | Mike Cope | |
| 88 | SealMaster Racing | Terry Cook | |
| 90 | L & R Motorsports | Lance Norick | |
| 96 | Liberty Racing | Kenny Martin | |
| 97 | Ron Barfield Jr. | Ron Barfield, Jr. | |
| 99 | Jack Roush | Kurt Busch | |
