| Teams 2000 Daytona 250 |
|---|
| No. | Team | Driver | Mfg |
|---|---|---|---|
| 0 | McGlynn Racing | Ryan McGlynn | Chevrolet |
| 1 | K-Automotive Performance | Dennis Setzer | Dodge |
| 2 | Ultra Motorsports | Mike Wallace | Ford |
| 3 | Team Menard | Bryan Reffner | Chevrolet |
| 4 | Bobby Hamilton Racing | Bobby Hamilton | Dodge |
| 6 | Woodland Racing | Rich Woodland, Jr. | Chevrolet |
| 7 | PF2 Motorsports | Morgan Shepherd | Chevrolet |
| 12 | Impact Motorsports | Carlos Contreras | Dodge |
| 14 | Circle Bar Racing | Rick Crawford | Ford |
| 15 | Billy Ballew Motorsports | Geoffrey Bodine | Ford |
| 16 | Team Rensi Motorsports | Jimmy Hensley | Chevrolet |
| 18 | Bobby Hamilton Racing | Joe Ruttman | Dodge |
| 24 | Hendrick Motorsports | Jack Sprague | Chevrolet |
| 25 | Impact Motorsports | Randy Tolsma | Dodge |
| 26 | MB Motorsports | Jamie McMurray | Ford |
| 27 | CJ Racing | Lonnie Rush, Jr. | Ford |
| 31 | Brevak Racing | John Young | Ford |
| 35 | Team Texas | David Starr | Chevrolet |
| 41 | TKO Motorsports | Randy Renfrow | Dodge |
| 42 | McCray Racing | Jimmy Kitchens | Chevrolet |
| 43 | Petty Enterprises | Steve Grissom | Dodge |
| 46 | Dollar Racing | Rob Morgan | Ford |
| 50 | Roush Racing | Greg Biffle | Ford |
| 52 | Ken Schrader Racing | Lyndon Amick | Chevrolet |
| 60 | Addington Racing | Andy Houston | Chevrolet |
| 66 | Phelon Motorsports | Rick Carelli | Ford |
| 72 | MacDonald Motorsports | Randy MacDonald | Chevrolet |
| 73 | Sonntag Racing | B.A. Wilson | Chevrolet |
| 75 | Spears Motorsports | Marty Houston | Chevrolet |
| 84 | Long Brothers Racing | Donnie Neuenberger | Ford |
| 86 | Impact Motorsports | Mike Cope | Dodge |
| 88 | ThorSport Racing | Terry Cook | Chevrolet |
| 90 | L & R Motorsports | Lance Norick | Chevrolet |
| 96 | Liberty Racing | Kenny Martin | Ford |
| 97 | RDR Racing | Ron Barfield, Jr. | Ford |
| 99 | Roush Racing | Kurt Busch | Ford |