| Teams 2001 John Boy and Bill 250 |
|---|
| No. | Team | Driver | Mfg |
|---|---|---|---|
| 1 | Ultra Motorsports | Ted Musgrave | Dodge |
| 2 | Ultra Motorsports | Scott Riggs | Dodge |
| 4 | Bobby Hamilton Racing | Bill Lester | Dodge |
| 8 | Bobby Hamilton Racing | Willy T. Ribbs | Dodge |
| 08 | SS Green Light Racing | Bobby Dotter | Chevrolet |
| 14 | Circle Bar Racing | Rick Crawford | Ford |
| 16 | Xpress Motorsports | Mike Bliss | Chevrolet |
| 17 | Hendrick Motorsports | Ricky Hendrick | Chevrolet |
| 18 | Bobby Hamilton Racing | Joe Ruttman | Dodge |
| 19 | James Murphy | Ricky Sanders | Ford |
| 20 | Joe Gibbs Racing | Coy Gibbs | Chevrolet |
| 21 | Shepherd Racing Ventures | Morgan Shepherd | Ford |
| 24 | Hendrick Motorsports | Jack Sprague | Chevrolet |
| 25 | Impact Motorsports | Phil Bonifield | Chevrolet |
| 29 | K-Automotive Performance | Terry Cook | Ford |
| 35 | PVR Motorsports | Clay Young | Ford |
| 43 | Petty Enterprises | Carlos Contreras | Dodge |
| 46 | Dollar Racing | Dennis Setzer | Chevrolet |
| 50 | Roush Racing | Jon Wood | Ford |
| 51 | Rick Ware Racing | Brian Rose | Chevrolet |
| 53 | Frank Ciopettinni | GJ Mennen, Jr. | Ford |
| 54 | Tim Woods | Tim Woods | Ford |
| 60 | Addington Racing | Travis Kvapil | Chevrolet |
| 61 | Team Rensi Motorsports | Vince Whitmire | Chevrolet |
| 68 | R & J Racing | Rodney Sawyers | Dodge |
| 70 | Jim Mills | Morris Coffman | Chevrolet |
| 72 | MacDonald Motorsports | Jimmy Hensley | Dodge |
| 75 | Spears Motorsports | Billy Bigley, Jr. | Chevrolet |
| 81 | Rick Ware Racing | Michael Dokken | Chevrolet |
| 84 | Long Brothers Racing | Nick Woodward | Ford |
| 86 | Impact Motorsports | Dana White | Chevrolet |
| 88 | ThorSport Racing | Matt Crafton | Chevrolet |
| 90 | L & R Motorsports | Lance Norick | Chevrolet |
| 91 | Rick Ware Racing | Michael Ritch | Chevrolet |
| 93 | Troxell Racing | Jerry Hill | Chevrolet |
| 99 | Roush Racing | Kyle Busch | Ford |