| Teams 2016 Rattlesnake 400 |
|---|
| No. | Team | Driver | Mfg |
|---|---|---|---|
| 0 | JR Motorsports | Cole Custer | Chevrolet |
| 1 | Jennifer Jo Cobb Racing | Ryan Ellis | Chevrolet |
| 02 | Youngs Motorsports | Tyler Young | Chevrolet |
| 4 | Kyle Busch Motorsports | Christopher Bell | Toyota |
| 5 | Athenian Motorsports | John Wes Townley | Chevrolet |
| 07 | SS Green Light Racing | Ryan Lynch | Chevrolet |
| 8 | NEMCO Motorsports | John Hunter Nemechek | Chevrolet |
| 9 | Kyle Busch Motorsports | William Byron | Toyota |
| 11 | Red Horse Racing | German Quiroga | Toyota |
| 13 | ThorSport Racing | Cameron Hayley | Toyota |
| 17 | Red Horse Racing | Timothy Peters | Toyota |
| 19 | Brad Keselowski Racing | Daniel Hemric | Ford |
| 20 | Austin Hill Racing | Austin Hill | Ford |
| 21 | GMS Racing | Johnny Sauter | Chevrolet |
| 22 | AM Racing | Austin Wayne Self | Toyota |
| 23 | GMS Racing | Spencer Gallagher | Chevrolet |
| 29 | Brad Keselowski Racing | Tyler Reddick | Ford |
| 33 | GMS Racing | Ben Kennedy | Chevrolet |
| 41 | ThorSport Racing | Ben Rhodes | Toyota |
| 44 | Martins Motorsports | Tommy Joe Martins | Chevrolet |
| 49 | Jay Robinson Racing | Wayne Edwards | Chevrolet |
| 50 | Beaver Motosports | Travis Kvapil | Chevrolet |
| 51 | Kyle Busch Motorsports | Cody Coughlin | Toyota |
| 63 | MB Motorsports | Mike Bliss | Chevrolet |
| 66 | Bolen Motorsports | Jordan Anderson | Chevrolet |
| 71 | Contreras Motorsports | Carlos Contreras | Chevrolet |
| 75 | Henderson Motorsports | Caleb Holman | Toyota |
| 81 | Hattori Racing Enterprises | Jesse Little | Toyota |
| 86 | Brandonbilt Motorsports | Timothy Viens | Chevrolet |
| 88 | ThorSport Racing | Matt Crafton | Toyota |
| 92 | RBR Enterprises | Parker Kligerman | Ford |
| 98 | ThorSport Racing | Rico Abreu | Toyota |