| Teams 2016 Drive For The Cure 300 Presented by Blue Cross & Blue Shield of NC |
|---|
| No. | Team | Driver | Mfg |
|---|---|---|---|
| 0 | JD Motorsports | Garrett Smithley | Chevrolet |
| 1 | JD Motorsports | Ryan Preece | Chevrolet |
| 1 | JR Motorsports | Elliott Sadler | Chevrolet |
| 2 | Richard Childress Racing | Austin Dillon | Chevrolet |
| 3 | Richard Childress Racing | Ty Dillon | Chevrolet |
| 4 | JD Motorsports | Ross Chastain | Chevrolet |
| 6 | Roush Fenway Racing | Bubba Wallace | Ford |
| 07 | SS Green Light Racing | Ray Black, Jr. | Chevrolet |
| 7 | JR Motorsports | Justin Allgaier | Chevrolet |
| 11 | Kaulig Racing | Blake Koch | Chevrolet |
| 12 | Team Penske | Joey Logano | Ford |
| 13 | MBM Motorsports / Garage 66 | Brandon Hightower | Dodge |
| 14 | TriStar Motorsports | Jeff Green | Toyota |
| 15 | Rick Ware Racing | Clint King | Ford |
| 16 | Roush Fenway Racing | Ryan Reed | Ford |
| 18 | Joe Gibbs Racing | Matt Tifft | Toyota |
| 19 | Joe Gibbs Racing | Daniel Suarez | Toyota |
| 20 | Joe Gibbs Racing | Erik Jones | Toyota |
| 21 | GMS Racing | Spencer Gallagher | Chevrolet |
| 22 | Team Penske | Brad Keselowski | Ford |
| 25 | Rick Ware Racing | Chris Cockrum | Chevrolet |
| 28 | JGL Racing | Dakoda Armstrong | Toyota |
| 33 | Richard Childress Racing | Brandon Jones | Chevrolet |
| 39 | RSS Racing | Ryan Sieg | Chevrolet |
| 40 | MBM Motorsports / Garage 66 | Timmy Hill | Toyota |
| 42 | Chip Ganassi Racing | Kyle Larson | Chevrolet |
| 44 | TriStar Motorsports | J.J. Yeley | Toyota |
| 48 | Chip Ganassi Racing | Brennan Poole | Chevrolet |
| 51 | Jeremy Clements Racing | Jeremy Clements | Chevrolet |
| 52 | Jimmy Means Racing | Joey Gase | Chevrolet |
| 62 | Richard Childress Racing | Brendan Gaughan | Chevrolet |
| 70 | Derrike Cope Racing | Derrike Cope | Chevrolet |
| 74 | Mike Harmon Racing | Mike Harmon | Dodge |
| 78 | BJ McLeod Motorsports | B.J. Mcleod | Ford |
| 88 | JR Motorsports | Kevin Harvick | Chevrolet |
| 89 | Shepherd Racing Ventures | Morgan Shepherd | Chevrolet |
| 90 | DGM Racing | Martin Roy | Chevrolet |
| 93 | RSS Racing | David Starr | Chevrolet |
| 97 | Obaika Racing | Jordan Anderson | Chevrolet |
| 98 | Biagi DenBeste Racing | Jeb Burton | Ford |